Sam Friedman Peroxsidase Enzyme Lab Report

Lab Report

Materials

• about a handful of bindweed leaves
• Mortar and pestle
• Distilled water
• glass or plastic beakers
• 1 mL syringe
• Hydrogen peroxide
• 1 Paper towel square (for filtration)
• Glass test tubes
• Test tube rack or holder
• Small plastic ruler
• Safety glasses

• Sodium Hydroxide
• Hydrochloric Acid
• Hot water
• Stopwatch

Hypothesis:  My hypothesis is that the acidic solution will speed up and increase the process, thus creating more bubbles.

Independent Variable:  The pH level of the solution and the amount that we add to the bindweed solution.

Dependent Variable:  The amount of bubbles produced by the mixture once the hydrogen peroxide is added.

Controlled Variables:  Amount of the different solutions and the acidity of them that we add.

Justification of hypothesis:  The acid seemed like it would eat away at the bonds making it take less energy for them to break apart.

Why did you choose this as your hypothesis?
We chose this because it sounded like it made sense.

Procedure:

1. Mash up one handful of bindweed with 30 ml of water in a mortar and pestle
2. filter using a paper towel into a separate glass, throw away paper towel
3. tape ruler, metric side, to test tube
4. extract 1 ml of bindweed solution into test tube
5. extract .7 ml of hydrogen peroxide into same test tube
6. observe bubbles and and time the rate of formation
7. record information
8. with a new test tube repeat steps 1-4
9. add .5 ml of hydrochloric acid to the bindweed solution
10. repeat step 5-7
11. with a new test tube repeat steps 1-4
12. add .5 ml of sodium hydroxide to bindweed solution
13. repeat steps 5-7
14. write out lab report
15. clean up lab space

Summary:  When we tested all of our different solutions, we realized that the control actually created the biggest reaction and the most bubbles.

Data and Results:

:

Our Control's results:
0 seconds - 2.5 cm
10 second - 3cm
13 seconds - 4 cm
22 seconds - 5 cm
36 seconds - 6 cm

Sodium Hydroxide Results:
0 seconds - 2.7 cm
6 seconds - 3 cm
13 seconds - 3.5 cm
84 seconds 4 cm

Hydrochloric Acid Results:
0 seconds - 2.7 cm
12 seconds - 3.5 cm
90 seconds - 4 cm

Conclusions:  My hypothesis was wrong neither added variable created more bubbles.  In order to conduct a better experiment, we could have tested a larger sample size of each of the solutions.  Unfortunately, we did not have time to do this.  However, I thought that our group worked effectively and conducted a viable experiment.  Of which the results were, adding acids and bases only hampered the speed of the reaction.