Peroxsidase Enzyme Lab Report

Materials

• 5 to 10 grams of freshly picked bindweed vines with leaves (about 1 handful)
• Digital balance (scale)
• Mortar and pestle
• Distilled water
• 3 100-liter glass or plastic beakers
• 1 mL or 5 mL syringe
• Hydrogen peroxide
• 1 Paper towel square (for filtration)
• Glass test tubes
• Test tube rack or holder
• Small plastic ruler
• Safety glasses

Depending on which environmental factor you choose to investigate, some of the following items will be needed for your experiment:

• Acid solutions with pH values between 2 and 6
• Alkaline solutions with a pH between 8 and 12
• pH test strips
• Ice
• Large plastic beaker (for ice bath)
• Hot water
• Large plastic beaker (for hot water bath)
• Thermometers

Procedure

This part will be determined by the students conducting the experiment. For an overview of the general scientific experimentation and research process, see the flow diagram below. Use the worksheet that follows to write out in detail the hypothesis you are seeking to test, the materials you will use, and the steps you will follow to conduct your experiment.

Enzyme Lab Worksheet

Hypothesis:

Independent Variable: The Different substances tested with the enzymes

Dependent Variable:Enzymes

Controlled Variables:Water (H20)

Justification of hypothesis:hypothesis: Because of knowing High and low ph levels that enzymes lose activity and we would like to see the reaction when you mix hydrogen peroxide with the different chemicals.

Why did you choose this as your hypothesis?

We wanted to test if ph level actually affects the enzymes.

Materials (Your Team’s Experiment):

● Beakers

● Syringe

● Test tubes

● Water

● Bineweed

● Ph 2­3 HC1

● Ph 4 HC1

● Ph 10 Na Oh

● Ph 12 Na Oh

Procedure: Using available chemicals, test the ph reaction with enzymes different solutions and hydrogen peroxide to see their reaction.

Summary:To test different ph solutions to see their reaction with enzymes with hydrogen peroxide.

Detailed Steps:

• label tubes
• measure out chemicals
• mash up bineweed
• pour water in
• squeeze out the enzymes
• pour enzymes into test tubes with chemicals
• add hydrogen peroxide
• measure reaction every 3 seconds
• gather results.

Data and Results:

(This section should include at least one graph!)

Ph 2-3 Hc1	Ph 4 Hc1	Ph 7 Water	Ph 10 Na Oh	Ph 12 Na Oh
0cm	0cm	0cm	0cm	0cm
0cm	0cm	0cm	0cm	0cm
0cm	0cm	0cm	0cm	0cm
0cm	0cm	.01 cm	0cm	.01cm
0cm	0cm	.01 cm	.01cm	.02cm
0cm	0cm	.02cm	.02cm	.03cm
0cm	0cm	.02cm	.04cm	.04cm
0cm	.01cm	.02cm	.06cm	.04cm
0cm	.01cm	.03cm	.08cm	.04cm
0cm	.01cm	.03cm	.08cm	.04cm
0cm	.01cm	.03cm	.08cm	.04cm

Conclusions:There was more bubbles when a higher ph level was included, while the lower ph level solutions did not affect the enzymes. So we can conclude that the higher ph solutions affect the enzymes in a bigger way than lower ph solutions.